Hello, I am preparing stock solutions of unialgal strains in a liquid medium. Which light and temperature conditions are the best to maintain these cells?
- Provide constant room temperature, or equivalent incubator, capable of maintaining temperature of 16 or 20 (marine) 22 or 26 (freshwater) in degree centrigrade unit. Using cool-white fluorescent lighting to provide 4304 lux+/- 10% or 2152 lux +/- 10% mesured adjacent to the flask at the liquid level with closure in place.
I agree with the answer of Chandan, also with interval shaking of culture.
Other important notes you have found it in the following book: Chapter 11 (Long-Term Macroalgal, Culture Maintenance by John A. West page 157), in Algal Culturing Techniques Edited by Robert A. Andersen, 2005.
Regards
Fikrat
I observed that some cultures we have kept in the dark for several months are still green and viable. They are inside an incubator at around 18°C but with no light inside, on a solid medium (agar). Do I have to expect a different behaviour in liquid cultures?
And another question, do I have to put my test tubes at light for a certain time at the beginning, to enhance the growth of my inoculum, and then move them in the low-light conditions, or can I skip this passage?
Yes, you have a different behavior in growth and color. And also you have positive respond in the 2nd case.
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