What are the best house keeping genes for RT-QPCR in undifferentiated murine bone marrow cells? GAPDH showed inconsistent expression?

28 February 2025 1 9K Report

I tried GAPDH and actinb, expression of both was inconsistent.

It’s best to validate multiple reference genes using a tool like GeNorm or NormFinder to ensure stability in your specific experimental conditions. Many researchers use a combination of at least two housekeeping genes for more reliable normalization.

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