How to assess the quality of specular highlight removal results?

Dear researchers, Recently, I have been working on detecting and removing specular highlights from natural and medical images. The obtained results seem quite satisfactory. However, given that...

03 October 2020 1,510 5 View

Interviews versus focus groups?

I am currently working on my doctoral research proposal. I am attempting to further justify using interviews versus focus groups to conduct qualitative research about a topic that is sensitive in...

27 July 2020 293 25 View

What is an appropriate sample size for qualitative research?

For my doctoral research, I will be conducting two parallel qualitative studies using interviews: one with persons with intellectual and developmental disabilities, and the other with family...

27 July 2020 9,055 11 View

Any observation of Bactrocera oleae controled by Macrolophus predator ? ?

Lot of Macrolophus this year on Bactrocera oleae in south France.

26 July 2017 6,782 19 View

What would be the best statistical strategy to compare two groups with a different number of subjects in each group (imbalanced design; 17 vs. 8)?

26 October 2016 5,138 13 View

Mitophagy ? how to stain lysosomes and autophagosomes differentially?

I have problem with somes markers... Did lysostracker Green or red only stain lysosomes. Or did this marker also stain acidic compartement as the AO did. I feel that AO staing is very efficient to...

27 April 2016 1,062 9 View

Which methods to extract parameters (amplitude, frequency and phase) of a sinusoid from a signal which is not sampled at equally spaced intervals ?

(no matter the application)

17 February 2016 9,908 4 View

Do you know the best methods/techniques and tools to test the theory of acceptance and use of technology?

I have extended the UTAUT2 model and I have already developed my model hypotheses. I read about various methods/techniques used such as (Factor Analysis, Partial Least Square (PLS), Structure...

04 February 2016 9,343 3 View

Where can I find export data and fdi data of airbus aircrafts and boeing aircrafts to China?

Thanks for your help !

28 December 2015 5,305 3 View

Is there a length of time when AA 12-step meetings cease to be beneficial for college-aged students?

I am searching for research supporting AA 12-step meetings during college years for those diagnosed with substance use disorders. Thanks.

11 December 2015 6,566 7 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View