I have understood that analyzing genotoxicity with the comet assay depends on which parameter I decide to use, and according to some other publications & scientists, some of them prefer one over another. But, I would really like to know what are those preferences based on.
I'm currently trying to determine the genotoxic effect from some synthetized molecules but I have differences in the results between these three parameters. I’m having trouble on understanding which one would provide me more accurate results or if it’s ok if they all are not related in those three parameters. I would really appreciate some guidance and responses.
In my case, I use both the tail moment and the tail length because they can act as complementary parameters. Because they do not show any appreciable difference and give support to the results, other authors prefer the moment of the tail because they count exactly the% of DNA damage, in the words of another author: "It remains linear in a wide range of damage to the DNA and from its value, it is also easy to imagine the magnitude of the damage ".
However, the use of tail length is remarkable because of its simplicity.
Agreed with Alexis Sámano León tail Length is basis parameter due to its simplicity.
The four major parameters which researchers commonly follow are:
1. Tail length
2. Tail moment
3. Olive tail moment
4. % of DNA in tail
The length of tail vary with the comet assay conditions : alkaline and neutral comet assays
you can add one more parameters Apoptotic comet if your genotoxic compounds is showing some sever damage upto apoptosis.
For that You can follow the article how comets consider as apoptotic comet depending upon the % of DNA damage occurs in the cells.
Article Sesamol ameliorates radiation induced DNA damage in hematopo...
Dear Ms. Ma. Cristina Cueva ,
Greetings!!
Please go through this link: http://www.cometassayindia.org/definitions.htm
Best Regards,
Samrat
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