Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Langevin Dynamics in shear flow?

Hi, We are trying to simulate particles with Langevin dynamics in a shear flow by Lees-Edwards boundary conditions in MD simulations. It seems thermal forces and shear flow do not go hand in...

23 February 2021 1,761 1 View

How can I homogenize this data to make a meta-analysis?

Hi, I am doing a review and hopefully meta-analysis for my Master thesis but I have the following problems: - Some studies only give the difference between end trial and baseline data and other...

22 February 2021 1,115 4 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

Is there any relevant technique for mesocosm studies in marine environment using Nanoparticles?

I am planning to perform mesocosm study in simulated marine environment using Phosphorus based nanoparticles, i would be using marine micro-organisms in it. kindly suggest me some effective...

07 January 2021 5,877 3 View

Which chemical analysis method is commonly used and efficient to determine organic carbon , inorganic carbon in pore water samples ?

In the framework of the ongoing research project, we will sample pore water from sediments in the mesocosms to investigate on carbon cycling within the system.

09 October 2019 6,755 3 View

Hello everybody, I'm confused, I want to know, which characteristics should have a mesocosm in lakes?

if I am going to check the filtration rate of little fish (10 cm) ...size, liters etc...If the mesocosm needs water flux or not, If somebody know reference about this topic, I will gratefully.

02 March 2018 2,186 0 View

Mesocosm transplant across env gradients -what are your thoughts?

Simulating change in ecological systems can take many forms. We are interested in making generalisations about ecological response to change, including climate and land-use intensity. We aere...

19 July 2017 6,195 3 View

Mesocosm transplant across env gradients -what are your thoughts?

Simulating change in ecological systems can take many forms. We are interested in making generalisations about ecological response to change, including climate and land-use intensity. We aere...

06 July 2017 9,982 3 View

Microbial Desalination

22 June 2017 1,018 13 View

What are the problems we could face during a mesocosm set up?

I am currently working on evaluating the effects of climate change on marine plankton using a mesocosm facility. I have some queries The capacity of my tank is 2000 L - Indoor facility 1. Do I...

01 February 2016 6,072 7 View

As a resistant protein, could glomaline measurements be used as a proxy of past AM activities/abundance in soil ?

01 February 2016 3,820 7 View

How can we interpret the increase in primary production in the toxic effects? Are there any publications on this topic?

In experiments on mesocosms, we found an increase in primary production as an early response to toxic effects. Then the depression of production. In what studies described this response of...

09 October 2015 9,936 0 View

What are good standard methods for calculating MPNs from sediments?

02 March 2015 9,008 5 View