Dear all,

I am about to conduct an experiment to determine IgM production in mice (5 days post-injection) of PEGylated particles. Since this will be my first time using the IgM ELISA Kit (Abcam ab133047), I would appreciate any suggestions or tips you might have.

I understand that the kit is quite sensitive, particularly with user handling, and I am unsure about the appropriate serum dilutions for accurate testing. I have the 96-well kit and cannot afford to waste wells due to incorrect handling.

Any advice on optimizing the use of this kit, especially regarding dilution protocols, would be greatly appreciated.

Thank you in advance.

Best regards,

Evgenia