Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

Cause of blisters in ALD layer at the edge of electrode?

I've encountered a defect during processing that I can't figure out, so I'm hoping I might find some ideas here. My mask design is a microelectrode array, with 60 microelectrodes with diameter...

19 January 2021 665 1 View

Difficult to silence gene?

I have been trying to silence a gene of interest but I could not get silencing efficiencies beyond 50% My method of detection is via RT-qPCR and samples were extracted 48hrs post transfection. Are...

09 January 2021 3,404 4 View

What form of data analysis should I use for my pilot study?

I am currently conducting my final year dissertation which is looking at the efficacy of common police lineup practices. However, before I launch my study, I have put out a pilot study which is...

08 January 2021 4,376 4 View

Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

Hello, to start off I attempting to test the bacteriostatic properties of certain compounds. I bought a kit that contains some of the equipment to perform this analysis, however, in the included...

02 January 2021 2,338 2 View

Cleaning SGE Micro Channel Device?

Hello all, I have a 3-port SGE microchannel splitter in my Thermo Trace 1310 GC connected to an Isolink 2 combustion interface. My microchannel device is clogged and my flow is significantly...

29 December 2020 7,002 1 View

Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Can R be used for SEO?

13 January 2021 1,920 1 View

Santolina species, wax moth and bees?

Expect a savage answer and being reported to ResearchGate Admin if your answer clearly shows you did not read the whole question and you are scamming the points system by answering with an...

12 December 2020 4,413 7 View

What was scientist thinking about Bees communication before Karl von Frisch discovery?

In the 1920s, Karl von Frisch pointed out that bees use special dancing patterns. What scientist or people think about bees communication before its discovery.

22 November 2020 1,426 5 View

LIFE in SMART CITIES: Animal data versus Human data, complementarity or not?

One observation is that in any city (those I know) you have humans, of course, but you also have many animals. Statistics of humans start with population data (for instance demographics) and...

08 November 2020 4,127 6 View

Is there a database of photos with insect pollinators on flowers of plants?

I would like to know if there is a database of photos with insect pollinators on flowers. The insects must be located on flowers of plants. I am interested in pollinators encountered in Europe,...

04 November 2020 2,219 6 View

Can anyone help me understand how to adjust my SAS glm analysis to include a random effect for site?

I am not great with statistics so please be understanding. I have 17 pollinator sites that I collected data from and am comparing the effect of habitat type (2 classes) on several different...

24 September 2020 3,140 5 View

How can I get all the publications of a single author?

To gather all the publications of a single author, two methods are described in the DBLP in the section of F.A.Q. But the problem is how to follow the precedure is notb well described. Can...

08 September 2020 5,284 5 View

Does anyone know of a 3D insect tracking system for a large environment (5x2x2m)?

Hi, I'm looking for a 3D tracking system to reconstruct the trajectories of flying bumblebees in a white environment with a few plants. The size of the environment is ~5x2x2m. I am struggling to...

26 August 2020 3,436 5 View

What is the best method for collecting and preparing pollen from insects stored in 70% ethanol, for visual identification?

Hello, I would like to visually identify and quantify the pollen morphotypes carried by insects which have been stored in 70% ethanol. The insects are Diptera (mainly Syrphids), and Hymenoptera...

20 August 2020 8,859 3 View