Hello Praise Emma-nwachukwu, you can go through this book "Antibiotics: Current Innovations and Future Trends" and article "Melding the Old with the New: Trends in Methods Used to Identify, Monitor, and Control Microorganisms on Cultural Heritage Materials"

It must be determined what the purpose of producing this microorganism is. for example, It may produce a specific enzyme or valuable molecule, but the bacterium or microorganism itself is pathogenic, so let us first identify the gene that produces the enzyme or molecule. The gene is extracted and placed in specific bacteria, such as certain E. coli strains with the PCR technique. We do this with the CRISPR-cas9 technique or using SnapGene software and genetic engineering primer design.

Many efforts have been made to increase the production of recombinant protein in E. coli genetically and on a genetic engineering scale. Protein expression in E. coli is the easiest way to prepare non-glycosylated protein for analytical purposes. Combined expression optimization depends on various parameters such as expression vector, culture medium composition, temperature, and expression chaperones. Good luck

I do not understand what you mean by the expression "producing microorganisms". These are biological systems just like human beings. Human beings are not produced. Neither are plants and other animals. Please us understand the real import of your question. Except it is a trend we intend to follow in future. If you desire to obtain microorganisms for antibiotic production, bioprospect (search) for them from a great diversity of environments. Future trend should concentrate on techniques to access the marine environment, techniques to isolate, in pure culture, more microorganisms with the functional genes that direct antibiotic synthesis. This should entail significant development in culture media, cultivation techniques arising from better understanding of the physiological, nutritional and environmental requirements of the organisms that bore the genes encountered in environments that hold promise. Regards!