01 January 1970 3 317 Report

What are some effective examples of automated red team modeling in cyber?

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How many tumor cells should I seed in a 96-well plate/well for virus infectivity?

The tumor cells are from a variety of cell lines from ATCC .

22 December 2020 1,950 3 View

How does band gap of a semiconductor shift with the lattice strain? and physical significance?

Someone posted a similar question but I have a further question on strain (increase/ decrease atom distance). So I know that the distance increases and decreases the bandgap distance. But can it...

04 June 2020 689 5 View

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance and yield attributing traits self pollinat?

Goodmorning..when doing genetic analysis of grain yield and yield attributing traits for drought tolerance in self pollinating crops (F2 generation) Vs cross pollinating crops, is it important to...

30 March 2020 1,011 3 View

What is the most appropriate statistical test for my experiment?

I am having trouble identifying the most appropriate statistical test for an experiment I am designing. In this experiment I am looking at multiple markers that indicate a change in structure...

25 November 2019 9,090 2 View

Where could I find some SOPs and formulations for making in house Clinical chemistry reagents?

Hi, I am looking for some resources to be able to find some SOPs and formulations for making in house Clinical enzymatic chemistry reagents like; Triglyceride, HDL-Cholestrol, Oxalate, Lactate,...

25 July 2019 698 2 View

Has anyone else had any problems with PSA antibodies for WB?

I am doing work on prostate cancer cell line - LNCaP - and using PSA antibody to show androgen deprivation. We currently have 3 in our lab, none of which are giving us a signal for western blots....

14 July 2019 2,723 3 View

When thawing cryopreserved cells, can they be added to 0.9% NaCl without loss of viability?

The cells are cryopreserved in 8% DMSO and 2% HSA.

08 November 2018 3,807 3 View

What is best way to check for non-proliferation in a tumor cell lysate?

We use 6x freeze-thaw cycles and consistently show 0% viability by Trypan Blue. We have used MTT and results were good, but some say DNA assays are better. Thanks

03 August 2018 4,119 3 View

What is second order CFA?

Hi ? I ran CFA in AMOS and fit indices were poor . I ran EFA in SPSS (etracted six factors) and delete some Items that were cross-loading , then run CFA again and now fit indices are within...

30 July 2018 9,679 5 View

Any antibodies against MMP2 and MMP9 that are suitable immunohisto?

Hello All. Can anyone suggest antibodies against MMP2 and MMP9 that work well for immunohisto in the mouse heart? Thanks in advance. Bruce

24 November 2016 7,284 3 View

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