What is the cause of bubbles formation DURING qPCR and how to mitigate it?

I am analyzing residual DNA in samples containing a high concentration of protein, therefore I have to do the extraction protocol. However, my results have been very inconsistent, ranging from...

18 November 2020 3,514 3 View

What is the best mitigation plan for school and community that sited to the river which is contaminated with chemicals released from nearby industry?

The river is classified as a category V contaminated river. Teacher claim that the bad smell often after and during raining.

26 July 2020 1,654 3 View

Could you anyone share the authentication code of blockchain?

I am working on cooja simulator for the Authentication of iot node with blockchain. could you anyone share the authentication code of blockchain?

30 January 2020 4,452 7 View

How to conduct UV vis spectroscopy of Cr doped ZnO?

We are to conduct UV vis of Cr doped ZnO but we are stuck in making solution... di water doesn't work... can we use HCl as a solvent for such transition metal doped metal oxides.. how about...

05 December 2019 8,397 12 View

Can anyone help me for the extraction of DNA from human saliva?

I have performed this experiment several times but failed.The main problem is that its UV spectra has a lot of distortion and its peak is not on 260nm. The DNA which i extracted has 1.5...

03 August 2019 4,614 3 View

How do I dissolve water in oil (w/o) nanoemulsion in water?

I did quick preliminary solubility study on my flavonoid enriched extract in oil and water phase. I found my extract is more soluble in water. Prior this study, I thought my extract is oil soluble...

17 July 2019 5,198 3 View

What are the companies producing active compounds i.e. flavonoids?

With regard to the question above, may I know any good companies are extracting, enriching or isolating natural active compounds i.e.flavonoids for pharmaceutical, nutraceutical or cosmaceutical...

08 May 2019 7,051 1 View

What if the absorbance value of plant extract is higher than the range of calibration curve?

I am currently doing research on total flavonoid content. I use Quercetin as standard for my calibration curve. The regression coefficient is 0.99. I use different solvent extracts as my samples....

30 January 2018 9,342 9 View

Why YbF3 nanopowder color becomes gray or black after sintering?

YbF3 powder color becomes gray/black when I sinter at 400C or 500C or 600C even when I sinter with a cover on the powder. I am using Yb acetate as a Yb source. When I use Yb nitrate as a source,...

17 January 2018 4,716 6 View

What is the optimum temperature for flavanoid plant extraction using sonication technique?

I am currently optimizing the temperature for my plant extraction. My interest is to extract optimum flavanoid using sonication technique. Currently I am setting the temperature at room...

12 November 2017 4,035 7 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View