I am using an organ bath instrument to understand smooth muscle contraction by metal pollutants, are there any new techniques to understand the same mechanism?
If you are using smooth muscle strips or rings the organ bath is fine. If smaller vessels there are myographs that can measure 100u blood vessels. There are pressurized vessel preps too. I'd keep it simple using the bath and generate a length-tension curve using potassium and then test your metals alone-to see if vasoconstrictive as some metals have been reported or force suppression. Then too you could use an agonist like angiotensin or endothelin and determine if metals affect receptor mediated contractions too.
thanks arthur chandler for your reply, please suggest me how can i study effect of metals and receptor mediated contraction. what are those receptors where metals effect the contraction of smooth muscle? i am studying effect of metal pollutant like nickel, cobalt , barium aluminium on smooth muscles. please send me some papers also.
An increasingly common technique used to measure smooth muscle contraction in the airways is the precision-cut lung slice technique developed by Mike Sanderson. It can be adapted to different tissues and has the advantage over organ bath contractions as the surrounding structural organisation of the tissue is intact. See this reference for further reading: http://www.ncbi.nlm.nih.gov/pubmed/21600999
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