Hi

Sachs et.al (2007) Published this article  ,Abstract attached .

I hope help you 

The Neutrophil-specific Antigen CD177 Is a Counter-receptor for Platelet Endothelial Cell Adhesion Molecule-1 (CD31)*

Ulrich J. H. Sachs ‡ 1 ,Cornelia L. Andrei-Selmer ‡ ,Amudhan Maniar ‡ ,Timo Weiss ‡ ,Cathy Paddock § ,Valeria V. Orlova ¶ ,Eun Young Choi ¶ ,Peter J. Newman § ,Klaus T. Preissner ∥ ,Triantafyllos Chavakis ¶ andSentot Santoso ‡ 2 + Author Affiliations

‡Institute for Clinical Immunology and Transfusion Medicine and the ∥Institute for Biochemistry, Justus Liebig University, Langhansstrasse 7, Giessen D-35392, Germany, the §Blood Research Institute, Blood Center of Wisconsin, Milwaukee, Wisconsin 53233, and the ¶Experimental Immunology Branch, NCI, National Institutes of Health, Bethesda, Maryland 20892

2 To whom correspondence should be addressed. Tel.: 49-641-994-1518; Fax: 49-641-994-1529; E-mail: [email protected].

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Abstract

Human neutrophil-specific CD177 (NB1 and PRV-1) has been reported to be up-regulated in a number of inflammatory settings, including bacterial infection and granulocyte-colony-stimulating factor application. Little is known about its function. By flow cytometry and immunoprecipitation studies, we identified platelet endothelial cell adhesion molecule-1 (PECAM-1) as a binding partner of CD177. Real-time protein-protein analysis using surface plasmon resonance confirmed a cation-dependent, specific interaction between CD177 and the heterophilic domains of PECAM-1. Monoclonal antibodies against CD177 and against PECAM-1 domain 6 inhibited adhesion of U937 cells stably expressing CD177 to immobilized PECAM-1. Transendothelial migration of human neutrophils was also inhibited by these antibodies. Our findings provide direct evidence that neutrophil-specific CD177 is a heterophilic binding partner of PECAM-1. This interaction may constitute a new pathway that participates in neutrophil transmigration.

Hi, we just made a SSC and FCS selection, after percoll isolation. However you must check the actiuvation because Neutrophils are very sentityve to almost everything. We evaluated activation markers like as CD11b and CD62L.

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Juan, Thank you very much for your answer. Would neutrophil retain their FSC, SSC pattern even after isolation? Also would the activated neutrophil have different FSC, SSC?