What are cosmids? - FAQS.TIPS

More Yamuna Daram's questions See All

The large odds ratio and the wide confidence interval, what are the potential reasons for these findings? Is this result reliable?

In my study, I found an odds ratio (OR) of 65 with a 95% confidence interval (CI) ranging from 1.2 to 195. Given the large odds ratio and the wide confidence interval, what are the potential...

07 June 2024 402 3 View

Can any please help me to calculate uncertainty measurement?

I have done some calibration tests and wanted to do some calibration calculations. So can anyone suggest me how to do uncertainty measurements for calibration? Any help would be appreciated.Thankyou.

10 November 2021 7,462 11 View

How to calculate the apparent viscosity vs apparent shear rate (rheological flow curve ) of polymers without any rheometers or viscometers ?

I am planning to find out the rheology using cross model. But, firstly, I should generate a flow curve of polymers with the mentioned parameters and without any help of instruments (rheometer,...

15 October 2020 6,992 3 View

Can anyone please help me with process of Acetylation ?? At what temperature and pressure it is performed and how the process is done ??

Acetylation is a chemical treatment done to natural fibers for less absorption of water. It is done by introducing acetyl groups in the functional group region.

02 August 2020 3,157 2 View

Why some mice have a very thin/not well formed skull?

In our lab, sometimes we find some mice that have part of the skull very thin, almost transparent, that it is clearly not well developed. This is particularly a problem when we do stereotaxic...

01 June 2020 8,395 0 View

What is the best way to measure the stock price?

I was working on a research, where I mainly willing to find the relationship between Corporate Governance, Earnings quality and Stock Prices. I was highly confused of measuring the share price at...

29 April 2020 4,185 6 View

How can a new modelling approach create a difference in the performance variation ?

How can a new modelling approach create a difference in the performance variation ? Please give some examples based on wireless communication scenario.

11 April 2017 580 2 View

How to solve a fractional program of unimodal nature?

I am trying to maximize an efficiency function which is fractional in nature, through power allocation. It is already known that the function has an uni modal behavior with respect to power...

04 April 2017 6,370 2 View

Has anyone worked in eigen value based spectrum sensing for cognitive radios?

The covariance matrix of correlated PU signals always gives the lowest eigen value as 0. Therefore, the ratio of maximum eigen value to minimum eigen value is always 0, which gives me a...

23 November 2015 748 6 View

How to determine water gradient or time to stop water with seedling stage, sandy soil and under drought stress for an experiment with Maize?

In field capacity, permanent wilting factor and water availability of plants are key factors, and so it is difficult to find the time to start water gradient with seedling stage. Experiment is...

28 April 2013 2,003 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View