What are Chronostratigraphic datums and events for Neogene palynomorphs?

02 April 2024 0 3K Report

I need to know the ages in million years for the Tortonian sequence pollens and spores.

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Trying to find a copy of an original paper?

Despite many attempts I am still unable to find the original version of this paper and would appreciate if anyone can locate it for us: Baker PG, Haig G (1981) Metronidazole in the treatment of...

20 June 2024 5,448 3 View

Microwave assisted Solid Phase Peptide Synthesis resulting in problematic 'sticky' gel peptide product following centrifugation of diethyl ether?

I'm trying to synthesis a hydroscopic peptide using a microwave assisted solid phase peptide synthesiser. I'm using an already optimised cleavage process, the issue arises after precipitating the...

15 June 2024 2,728 2 View

Can you use different Likert scales in the same questionnaire?

I am designing a questionnaire and am using a mixture of multiple choice and Likert scale questions. For most of my questions, a 4-point Likert scale is the most suitable but I have two questions...

03 March 2024 8,939 6 View

Substitution YES/NO ?

Dear all, I'm wondering about a possible substitution of a G>A base or a deletion of the G base in view of the chromatograms I've analyzed. I would like to point out that I have observed the...

21 November 2023 8,904 2 View

Do DNA diffuse from agarose gels?

how fast do DNA and RNA fragments diffuse from an agarose gel?

15 August 2023 7,142 5 View

What does theses chromatogrammes says?

Dear Sir/Madam, I am looking for a deletion or insertion anomaly in the CALR gene. After sequencing the same sample (Forward/Reverse), here's what I get. From the chromatograms we deduced that...

09 August 2023 4,907 4 View

Can you modify BSA with NHS-PEG4-azide?

I performed a reaction to modify BSA through esterification, but it didn't seem to work. Most of the papers that I've found that attach azide to BSA seem to use maleimide. Is there a reason why...

09 March 2023 4,971 2 View

Can you elute a protein from a Coomasie stained electrophoresis gel PAGE SDS?

I'm wondering if the methanol and acetic acid impedes the elution of proteins in an electrphoresis gel. I know methanol fix proteins, but do this is a permanent fixing? and what about the coomasie...

08 February 2023 4,969 3 View

Can I get assistance to formulate a PHD research topic with key interest in Big data and machine learning?

I wrote my innitail proposal on the Data quality and data cleaning in big data but I will like to incorporate machine learning in it. I would appreciate your suggestions on a suitable topics that...

07 February 2023 9,166 1 View

Who understands the transformation going on in Web of Science?

It is like Web of Science is merging with other indexing houses. Please share if you have full details of goings on there

17 August 2022 3,522 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

What is the best method to detect the chemical composition of pollen ?

I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?

28 July 2024 5,331 1 View

Can I use soybean, sunflower, or olive oil to prepare a standard curve for lipid estimation in plants or pollen?

I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...

01 July 2024 3,267 1 View

I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method?

I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method other than cryopreservation of spore for a short term...

10 June 2024 4,992 1 View

What is the correct way to measure the lengths of curved spores?

Measuring the lengths of curved spores sometimes makes confusions. Here in the attached image I have marked two paths (i, ii) the length can be measured. Hope an expert can suggest the correct way.

08 June 2024 1,109 5 View

How can i use hexamethyldisilazane(HMDS) for drying instead of Co2 and freez drying for SEM analysis of rice anther and pollen?

could you please help with a protocol? should i do it directly on the aluminium stub after dehydration with 100% alcohol or not? should i release the pollen or whole anther is better and dehisce...

03 June 2024 4,793 8 View

What are sources of negative control/artificial urine that I could use use dilute standards down in an ELISA?

Hello! I'm hoping to run an ELISA on human urine samples to measure testosterone levels. We are hoping to use a kit from Neogen, which has been validated for serum/tissue/urine. The protocol...

28 May 2024 2,815 0 View

Methodology for quantifying particulate matter on leaf surface & Pollen by Scanning Electron Microscopy with energy dispersive X-ray spectroscopy?

I needed a detailed methodology for SEM-EDS analysis for Leaf and pollen!

05 May 2024 4,892 5 View

Dear， How to define sexual spore?

I found it very difficult to define it precisely. Microspore and megaspore of ferns or seed plants are sexual spore? Because the fertilization happened for a long time before the formation of...

27 April 2024 3,077 0 View

Dear RG community, can you help us to identify these palynomorphs?

Hello, These NPPs were found in modern soil samples from the central Tunisian Dorsale mountain range. NPP6, 7, 8 and 9 seem to belong to Glomus-type. Could anyone provide a more precise...

18 April 2024 5,600 3 View