Deravative spectrophotometry is very useful to seperate signal of analytical compound from background or other compounds' siganal. Besides, it also makes peaks sharper and easier to take the information.
Except for two advantages, what are benefits and backdraws of this method?
Compared to conventional UV Visible spect
Signal to noise ratio - more, high selectivity, specificity, more precise, accurate, robust, LOD - good, LOQ good, linearity also will be good with good range
Compared to HPLC
selectivity, specificity, precision, accuray, robustness will be very less. LOD, LOQ, range will be good in HPLC compared to derivative spectrometery
fianly derivative spectrometry beter than UV Visible and not beter than HPLC
for better answer you need to wait for Dr.John.
Regards
Selvan (India)
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