Why does DMSO become liquid with extended storage at -20C?

I noticed that drugs or reagents dissolved in DMSO and stored at -20C become liquid after a year or two of storage. Does anyone know why this is and if it affects the effectiveness of the...

30 September 2020 7,901 2 View

How do I deal with small sample size and nonnormality in SEMs (AMOS or R)?

Hello! I have a sample size of N = 70 at the moment, hoping to aproach 100. My Model has 21 parameters to be estimated. The Variables are all not normally distributed. Now the options that I know...

20 July 2020 9,849 6 View

What is the Full Spectrum of Neuroscience Research Techniques that Exist?

Hi! I graduated with a Neuroscience and Psychology BSc in 2016, and my final year project was a bench-lab in vitro study of omega-3 oils applied to fluorescently-stained cortical neurons. I...

26 May 2020 4,650 6 View

Which cell culture inserts do you use?

In our experiment we use TC treated 96-well plates from Eppendorf and coat our protein of interest. After coating we add monocytes and get our readout. Now, we want to add activated T cells to the...

02 March 2020 6,065 2 View

What is the most efficient way to exchange buffer in protein soltuions?

Hy everyone, I'm facing some troubles when changing the buffer of my protein solution. I'm doing a 2-step purification of my protein, first on a nickel-column and second on a heparin column....

31 October 2019 793 4 View

Why does dynamic surface tension of gelatine solution increase before it decreases?

02 December 2018 949 3 View

How do I deal with recombinant protein seperation after MBP Factor Xa cleavage digestion of recombinant fusion protein?

After purifying my recombination fusion protein sample using a Bio-rad Q-column chromatography, the resultant samples, were verified via SDS-PAGE analysis and stored in glycerol 20% concentration...

29 July 2018 5,888 3 View

Why are there a huge number of dead cells in my culture ?

Hello, I am culturing 293 cells for onward transfection. Unfortunately, I have observed that the cells are not multiplying as fast as they used to. Besides, i see several dead cells in the T25...

26 June 2018 4,274 5 View

Why does my SDS PAGE gel bubble and form a bell shape towards the bottom of the tank?

Hello, I am doing some SDS PAGE. Now, i am baffled by the fact that my gel bubbles at the bottom and there is a bell shape in the sample under separation, towards the bottom of the gel ( as...

15 May 2018 8,675 2 View

What is the best method to assess viability for adherent breast cancer cell lines?

For my project, I need to set up a viability method to see if inhibitors are responding. We currently don't know which method suits best and because there are so many we don't know which one we...

04 April 2018 2,737 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Can I measure absorbance efficiency with wavelength in a spectrophotometer?

I measure the wavelength of material in a spectrophotometer and it peaks at 275 and its absorbance is 100. I mix it with an adsorbent and it adsorbs the first material, but when I measure the...

16 February 2021 7,515 5 View

How much costs direct reduction of nitrogen on farmland in terms of lower crop yield?

Dear research fellows, I wonder if someone knows how much direct reduction of nitrogen on farmland costs in terms of lower crop yield expressed as EUR/kg nitrogen (or similar)? I understand that...

15 February 2021 1,323 3 View

Are there alternatives to the Devarda alloy when estimating nitrogen?

Are there alternatives to the Devarda alloy when estimating nitrogen? devarda alloy is reducing the nitrate to ammonium

15 February 2021 8,838 3 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View