What alternative reagent can one use for free radical scavenging in the absence of DPPH?

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Vusi Mshayisa

Alok Nahata

You can use NBT (Nitro Blue Tetrazolium) assay which is also widely accepted and measures the superoxide radical scavenging activity of samples.

Efere Martins Obuotor

ABTS will do. However, i think from your location (Nigeria) it may be pretty easier to procure the DPPH reagent than the ABTS

Dr. Tresina Soris P

Respected Sir,

You can proceed your analysis using Superoxide radical scavenging activity, Hydroxyl radical scavenging activity and ABTS radical cation scavenging activity. Kindly go through the attachment.

Good Luck