CRISPR/cas9 is the future now of gene editing technologies, it also includes different versions, of activation, interfernce ..etc.
usually you start with designing guide rnas followed by PAMs which could be done computationally, then you use a plasmid vector for expressing Cas9 and single-guide RNA was mobilized into your target cell.
then you would look for mutation induced by cas9 or whatever you had used. Kits prices varies according to your query whether it's a plasmid, a knockdown kit or just a starter kit.
Crispr cas9 inducing DNA double-strand breaks is the most efficient method of genome editing with low cost and low cytotoxicity
Crispr cas9 workflow
1. Design and synthesize gRNA
2. Transfert cells
3. Determine frequency of successful edits
4. Establish single –cell clones
5. Screen clones for edit
6. Characterize successful edits
Available bioinformatics tools for CRISPR/Cas9
E-CRISP (http://www.e-crisp.org/E-CRISP/)
Genome engineering resources (http://www.genome-engineering.org/)
RGEN tools (http://www.rgenome.net/)
ZiFitTargeter Software (http://ccg.vital-it.ch/tagger/targetsearch.html )
CRISPR Design tool (http://www.broadinstitute.org/mpg/crispr_design/)
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