Generally, in FCS, the changing viscosity is taken care with by doing control experiments with some dye (like r6G) at each experimental point. My query is when studying some biomolecule, like protein, is it still ok to correct the viscosity in this way? The drag experienced by a small rhodamine molecule and a big full protein may not be similar because of surface area difference of the two. So, Does this create problem and what can be done regarding this
Instead of using the small molecule rhodamine, how about using a macromolecule of size comparable to your experimental molecule ?
Check in literature whether there exist some mechanistic model that relate viscous drag to the macromolecule size, if not i suggest you try to build such emprical model based on data driven statistical modeling, for example Partial least squares based models or on a simpler level, ordinary least squares based model.
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