Viability of NALM6 cells is below 85%. How can I increase viability? I have used proper cell media.
Hi Jiandong. I have not used these cells but I have grown other B-cell lines.
It is quite common for suspension cells to have viabilities between 80-90% (as unlike adherent cells you can't easily "wash" away dead cells in the media - which contribute to the lower viabilities). This is especially true when they have just come out of frozen.
You could try pelleting the cells and resuspending them in fresh media - this will remove the smaller, dead cells. I typically do this prior to setting up experiments (which I don't like to perform with viabilities lower than 85% for non-adherent cells).
I would not worry too much unless the viability falls below 80% (or continues to decline).
These cells do appear to have a rather narrow "window" for optimal growth of between 1-2x10e6 per ml and should not be allowed to exceed 4-6x10e6 per ml
https://www.dsmz.de/catalogues/details/culture/ACC-128.html
Sorry I can't be more help!
Gary
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