Hi,
Can anybody help me to figure out this below error during running pvacseq?
Exception: Leading amino acid of the Downstream protein sequence (NYEEEKILSHSETNVIPSGNL) expected to match the wildtype amino acid at postion 298 (M). You may need to reannotate your VCF with a newer version of VEP.
I have two vcf files. one vcf file is working fine, no error while other one giving this error. So, VEP may not have any issue. I have checked the github community for this error but nobody has actual solution. everybody refers to VEP. But how come when anothe vcf file iw wotking well and while other not with the same version of VEP. my current version of vep 101.
I'm using GRCH37 genome assembly.
Please help me urgent. Thanks.
Best,
Shams