I will be freeze-drying small skeletal muscle samples (~60mg) per sample using a Virtis SP Scientific Benchtop Pro Freeze Dryer. I've had difficulty findings a reference protocol to determine the optimal length and would love input. There is seemingly plenty amongst various animal models using larger samples (200-300mg) running them for ~24h, but I'd like to save time if possible.

Has anyone seen any such methodology and with the above device if possible?

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