Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Desalting using DSC- 18 SPE columns?

I am looking forward to remove excess of salt form my metal based porphyrin and trying DSC-18 columns for the same, can someone suggest me how how can one decide the polarity of the solvent to...

01 February 2021 9,076 1 View

Can we use two antibodies generated in same species for indirect Sandwich ELISA?

I have generated two antibodies against two peptide fractions of a protein in the rabbit. I want to use these two antibodies (Ab-1 and Ab-2) in indirect sandwich ELISA for the detection of...

11 January 2021 1,754 4 View

I want to quantify disturbance for wetland birds?

I have categorized the disturbance basically on movement of people and vehicle in into 3 categories. Disturbed, close to highway and movement of people from dusk to dawn. Intermediately...

30 December 2020 8,806 4 View

I am amplifying my target region through PCR, but I am getting number of unwanted band also with my target region...Why?

I have checked annealing temperature through gradient PCR. I have checked my primer in NCBI. I have checked PCR reagents concentration. Please suggest me anyone thanks

12 December 2020 1,969 2 View

What is the relationship between the type of material(ex: steel, iron etc.), thrust and torque?

If I am drilling through bars of different material(ex: iron, steel etc.) of the same thickness and under the same conditions of drill operations(feed rate and rpm), how will the torque and thrust...

06 November 2020 4,498 3 View

How to coat plasma sample (Antigen) in ELISA plate for a direct ELISA?

I have generated an antibody against an antigen. Now, I want to use the developed antibody in the detection of the unknown sample. So, I want to coat the plasma sample as antigen. How that can be...

26 October 2020 2,259 7 View

How the geospatial variability in the heat requirements of crop due to rising temperatures analyzed?

As we know that the global temperatures are increasing due to climate change which could affect the thermal needs of the crops. I want to make geospatial maps and analysis for heat requirements of...

28 September 2020 8,286 2 View

Some issues regarding electrical simulation of a Nanoowire based MSM Photodetector using Synopsys Sentaurus TCAD ?

I am simulating a NW based MSM Photodetector with Si/ CdTe/InAs NWs (modeled in SDE)with moderate doping, and have assumed a constant generation rate in the the Physics Section of SDEVICE 1....

03 September 2020 5,971 10 View

Can any body suggest the funding agency for Research Project on climate change and Remote Sensing?

I tried to submit research projects on climate change and Remote sensing to many agencies but could not get it though? Is there any easily available agencies who support the research in the subject.

16 August 2020 4,000 18 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Why ICP-MS test for Teflon filters that collected PM2.5, show really high results in the millions of ug/g ?

I analyzed soil and ash samples using ICP-MS for 28 metals, then I resuspended those samples to collect PM2.5 particles in a filter using a selective inlet inlet. My only issue that Fe and Al...

13 February 2021 7,592 3 View