I think that you have to use the ethanol as blank to calibrate the measurement.
Seeing differences in peaks does not necessarily mean that there is a reaction. Even comparing the same compound in two different solvents can result in a different number of peaks and/or splitting of one peak into several due to solvent interactions having an effect on the electronics of a molecule. To know if it is reacting you should try to find another way to analyze it like NMR, IR, or a chromatography method of some kind. Or remove all of the solvent and collect a new UV-vis of the solid to compare to the initial scan.
Thank you everyone for the reply. The molecule has C1 symmetry. It is an enantiomer. It has a benzene ring and an aliphatic chain is attached to it. In the aliphatic chain, a secondary alcohol and a secondary amino group is present. In solid phase, we get only one peak at 264 nm, while in ethanol solution it is 253 nm and 224 nm. They are experimental values. Also, the theoretical values using gaussian agree with the experimental values
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