QPCR troubleshooting - what could cause an increasing ROX signal?

Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....

08 February 2021 419 3 View

How to isolate RNA in a way that is representative of an entire brain (fish)?

Hi there, I'm looking to compare the expression of a particular gene in fish brains across three developmental stages. My aim (as first step) is to use the entire brain for RNA isolation steps....

02 September 2020 7,195 3 View

Author unable to be contacted prior to publication?

Can anyone let me know what to do in the case of a key investigator being uncontactable at the time of manuscript submission. Most journals ask for a signature and address of all authors and yet...

25 January 2018 1,627 3 View

Can I load samples into an electrophoresis gel, let sit overnight, & then turn the power supply on in the morning (and the gel run smoothly)?

samples (from yeast)= Whole cells, spheroplasts, crude mitochondria, and isolated mitochondria

24 August 2017 3,677 16 View

Trouble with Phosphorylation/Ligation of a blunt-ended CRISPR plasmid. Any troubleshooting ideas?

I have been trying to phosphorylate and ligate a blunt-ended linear plasmid after PCR. It remains linear. Any suggestions for troubleshooting? I have tried 10 min at 70 degrees followed by ice for...

15 March 2017 3,701 3 View

How do I do hierarchical cluster analysis in STATA on 11 binary variables?

I have 11 sleep outcomes - all binary .i.e. initial insomnia? yes/no. I want to see whether the 126 individuals I have cluster onto the different sleep variables. I have tried EFA before, and the...

28 February 2017 9,569 3 View

I'm trying to silence a microRNA using an antagomiR and lipofectamine2000 and have not been successful so far. Does anyone have any tips?

19 June 2016 5,496 6 View

In C57/Bl6 immunisations/challenges, are there any differences in using CD95 or GL7 positivity as a marker for germinal centre B cells?

06 October 2015 2,298 2 View

How long can I incubate C3H10T1/2 cells (or 3T3-L1 cells) with cell permeable PKA inhibitor 14-22 amide?

And should I incubate them at 37C or room temperature? If at RT, should it be in the dark? I am differentiating the cells into adipocytes, using the MDI protocol (IBMX, dexamethasone and insulin)...

30 September 2014 8,930 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is better medicine or food in obesity?

Preference in terms of the scientific aspect

28 February 2021 796 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

What are damage parameters for SMA while modelling on ABAQUS 2020?

I have given Elastic and Superelastic material properties, but I think we need to provide the damage parameters also. Please help me here.

16 February 2021 8,694 1 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View