Hello, this was an unexpected hurdle, I thought using the AngioTool software would be quite straightforward, especially given the scarcity of sources explaining its use and troubleshooting. But even after increasing the contrast and the hue of my pictures to make it as close as possible to clear lines, it still insists on considering the edges of the blood vessels as separate vessels, thus doubling the number of vessels on the CAM. I tried playing with all the available metrics, but I still can't find a way to make it count the single vessel as it should. Any suggestions?