Using paraquat with whole phytoplankton cells?

31 May 2019 1 675 Report

I am trying to use paraquat (methyl viologen) to test a hypothesis related to my research on chlorophyll fluorescence. Most of the literature on paraquat that I've seen (at least to study photosynthesis) uses thylakoid suspensions. I'm not having much luck using it on whole diatom cells and I was wondering if anyone had any advice.

Matthew Brown

So after some experimenting I discovered that an incubation time of at least one hour was necessary to see results. After that, fluorescence didn't show any further change. I'm leaving this answer for any who has the same question I did.

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