I would like to know how old can the fungal fruiting body be for detection and screening of antioxidants. Has anyone tried this with herbarium material? Does the preservation method affect the contents?
In my opinion, It will be nicer if you can do the antioxidant activity using new samples; we do not know whether the preservation affected (the stability) of the chemicals content .
It really depends in the preservation method. In vascular plants and pteridophytes many samples are collected and preserved in ethanol before drying (we do that mostly in tropical regions). That greatly alters the chemical composition. Drying of course (if too hot or too slow) will also have an effect. In case of fungi that are air-dried or lyophilized I would think the effect will be minimal.