Hello!

I would like to dissociate, antibody stain, FACS, and then prep RNA from mouse brain. The trick is that the antibody target is a cytosolic protein, so the cells have to be fixed before I stain them. I usually use PFA to fix, but this isn't compatible with having useful RNA as a product of the protocol.

I've come across a few papers describing the use of DSP (apparently aka lomant's reagent) which is a 'reversible' fixative. I'm going to give it a go, but was hoping to ask if anyone:

1) Has a better way to do this that they have done successfully (bonus points for a reference/protocol)

2) Have experience using DSP or it's soluble cousin DTSSP (found on ThermoFisher website)

3) Have a protocol for using DSP/DTSSP to do something like this

Thank you so much! For ref, I've seen these papers already:

- Attar et al 2018 Sci Rep 8:2151

- Xiang et al 2004 Nuc Acids Res 32:22