Using Complete freund adjuvant that was stored in -4 in EAE

01 January 1970 0 6K Report

I would like to induce EAE in my mice however I only have 10mg/ml solution of CFA that was prepared in February. It was stored in 4 degrees since then, is it still okay to use to make my MOG- CFA emulsion?

Badges
Science topic

More Aaliyah Hay's questions See All

How do I get ResearchGate to remove citations to things I have NOT written?

There is no obvious link on the website to allow correction of errors by ResearchGate or myself.

17 May 2023 4,339 2 View

Cryosectioning Brain Tissue- Issues of OCT separating from tissue?

Hi, Im having a hard time getting good sectioning on my PFA fixed tissue. No matter how I try to cut my sections, brush method or anti roll plate, my tissue is coming apart from the OCT which...

27 March 2023 9,845 2 View

Does anyone measure the chemokine and cytokines in patients' FFPE tissue slide by using immunofluorescence assay?

If the tissue is old tissue, can these soluble factors be measurable?

08 April 2022 9,024 0 View

What should I consider when I want to calculate the sample size of cohort?

I am proposing the cohort that will search for a predictive biomarker for treatment response. I used the formula using 95% CI and 0.05% error. And I used p as the response rate from the previous...

09 August 2021 5,208 3 View

COMET assay gone wrong, how can I interpret these results?

I've performed a COMET assay multiple times, improving on the procedure each time and re-making my solutions, but continue to get the same inconclusive results. My supervisor and I both tried...

06 February 2019 7,932 3 View

Can DAPI be used to stain for a COMET assay?

I am struggling with getting quality results from my COMET assays, and have been using DAPI as a stain. Is there any reason DAPI can't be used? I'd rather not use EtBr but will if DAPI is the issue.

06 February 2019 4,066 6 View

What are good examples of shifts in symbiotic relationships (e.g. parasitism to mutualism) between two species?

I came across this research paper stating the positive and negative effects of Branchiobdellidans on steam crayfishes (Ames 2013). Although their relationship shows a shift from parasitism to...

11 May 2017 7,455 2 View

Can EDTA be injected directly into the tissues of plants instead of the soil to aid in the phytoremediation of heavy metals?

We are currently working on a research on phytoremediation of heavy metals by plants, and our research uncovered that adding chelating agents, such as ethyldiaminetetraacetic acid (EDTA) increases...

19 April 2017 8,982 4 View

How does heterozygous Single Nucleotide Polymorphism, but not homozygous ones, induce a disease? what is the biological mechanism to explain this?

IL10-592 CA genotype, and not the CC nor the AA genotypes, has been found to be associated with development of the disease. How can we explain this finding?

09 May 2016 8,745 4 View

Is there a credible estimate of the number of Zika cases before the recent expansion into Brazil and the America's?

I would be grateful for any good sources

26 January 2016 3,762 7 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View