Using camera to align the laser spot onto the cantilever and the sample near the cantilever in atomic force microsopy ?

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Saad Zahraw Sekhi

You can read the following links:

1. https://hal.archives-ouvertes.fr/hal-01871339/document

2. https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Physical_Methods_in_Chemistry_and_Nano_Science_(Barron)/09%3A_Surface_Morphology_and_Structure/9.02%3A_Atomic_Force_Microscopy_(AFM)

3. https://cleanroom.utdallas.edu/manuals/tapping-mode-atomic-force/

Valeriy Sterligov

Ayman Ghwini

>Depth of field, the focal length of the objective and the working distance.

The cantilever oscillation amplitude is in the range of submicron or micron, so for the 8 mm focal distance lens, you can neglect it.

>Since the view followed by the dichroic mirror, it is difficult for me to understand, what depth of field should I use and how to calculate it in case of a dichroic mirror?

About dichroic mirror, there are several aspects. Dichroic means only spectral dependence of reflection/transmission ratio. So one spectral range, say laser one, will be mainly transmitted, while white light will be mainly reflected.

In the layout you presented, a dichroic mirror is unnecessary because of monochromatic laser light. It can be a conventional semitransparent mirror.

From the other side, there is no white light source, which will illuminate the sample, cantilever to correctly arrange the measurements. With such additional illumination application of a dichroic mirror is practical. It should decrease the influence of such illumination on the photodiodes signals.

>Further more a light source would be necessary to have a good view using the camera. Should I use a condenser to distribute the light evenly in the cantilever area ?

Yes, I mentioned such an illuminator above. Is the presented layout fixed, or do you have the possibility to modify it? It is not a strong requirement to obtain a homogeneous distribution of white illumination light. It can be used even image of the white light source (lamp or LED) on the sample, cantilever area.

>How can I make sure that footage taken by the camera is not white ?

I don't understand your question. If you use a correct dichroic mirror, the camera will image a white illuminated sample with a cantilever and a red laser spot on it.