Hello Ghadireh Mirabolghasemi

You should optimize the PHA-M concentration to ensure the desired outcome in the mixed lymphocyte reaction with PBMCs. You should perform dose-response experiments by testing a range of PHA-M concentrations (e.g., 0.5, 1, 2, 5, 10 µg/ml) to identify the concentration that induces the desired level of lymphocyte proliferation without causing excessive toxicity or inhibition.

PHA-M is typically used at a final concentration of 1-5 µg/ml to stimulate lymphocyte proliferation.

Best,

Hello Malcolm Nobre, thank you for your assistance. However, the sample of PHA-M I have is in liquid form, and the weight is unknown.