Regarding Fluoroscence readings?

I am running an enzyme assay in Perkin Elmer Victor 2D fluorometer. The device displays its results in units called "Counts". I have run a set of methylumbelliferone standards and recorded the...

13 July 2020 7,839 2 View

How to draw a conclusion that certain binding energy of an element in XPS data relates to particular oxidation state?

How to draw a conclusion that particular binding energy value of an element in XPS data relates to which oxidation state of that particular element? For Eg. Co2p3/2 and Co2p1/2 peaks at ~792.6...

21 April 2019 4,438 3 View

How does applied pressure affect the ceramic pellets? should the pressure be high for obtaining high dense pellets and crack free or vice versa?

How does the applied pressure affect the ceramic pellets to be crack free. My pellets when pressed are crack free, but after sintering I find the pellets crack. what would be the reason?

23 October 2017 5,815 6 View

Is it possible to find individual contribution of some 200 plus problems to the sum of time taken to solve all the problems?

I have an excel sheet with the number of instances for a particular problem in a day and the total time taken to solve all of the problems in the day. I have data for the previous 2 year. Is it...

03 October 2017 8,409 1 View

Can anyone recommend an ion-exchange column and a gel filtration column for protein purification?

19 October 2016 9,164 3 View

What are the applications of UHMWPE in automobile sector?

29 July 2015 3,469 3 View

What is the control and blank in the total antioxidant assay or phosphomolybdate assay for plant extracts?

25 March 2015 8,802 1 View

Could anyone suggest the total treatment period and sacrifice time in acute inflammation ( in-vivo study time duration of paw edema in rats)?

could any one suggest me a good article which carries the treatment period and after how many hours should i kill the rats by cervical dislocation protective effect of medicinal plant in formalin...

08 February 2015 4,658 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View