Dear Sandeep

I am from a human IVF background, and the culture seems to be contaminated; it could be culture media contamination or process-mediated contamination.

Kindly check your media and dishes, and other disposables and try to start the batch with fresh media and disposables. It should resolve the problem of the arrest.

If the strategy is not working out, you may have to look into the stimulation protocols and media quality assurance.

Regards,

Sanketh Dhumal Satya