Dear Tasmia Minhas
Probably, a quality of your sequence is low.
Try to download e.g. FinchTV free software tool. Than you can see your sequence. Using this tool, you can also edit each nucleotide base based on the colour of the peak, if there is any.
Good luck,
Marcela
Thank You
I would reconsider to re-sequence my sample. Questions mark are indice of low sequence quality and is not trustable.
Best
Loubna
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