How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

Is it possible to remove the mucus covering a fish's tongue once fixed in formalin 4%?

Hello everyone, Is it possible to remove the mucus covering a fish's tongue once fixed in formalin 4%?

29 November 2020 2,580 1 View

Simulator for Wireless sensor network?

I am looking for a wireless network simulator to test a query classification algorithm. I have already tested NS-2 (https://ns2simulator.com/ns2-download/), It's very complicated

18 November 2020 5,383 6 View

What are the factors that can improve the open circuit voltage of organic solar cell?

20 September 2020 8,237 5 View

How to reduce the series resistance and increase the shunt resistance of organic solar cell?

Hi dear colleagues, I am fabricating an organic photovoltaic device with the structure ITO/PEDOT: PSS/ P3HT: PC70BM/ Al, and the obtained IV relationship was found to be linear rather than...

19 August 2020 4,945 5 View

Is it possible to detect XRD spectrum to a thin layer (about 50 nm) of ZnO spin-coated on glass substrate?

I am trying to get ZnO thin film by spin coating on glass substrate, the procedure below was followed: 1- Mixing 0.109 gm of Zinc acetate dihydrate (> 99% )with 32 micro Litre of ethanolamine...

29 June 2020 9,035 28 View

Are there any modern papers about small hydro-power stations or run of river station?

dear all I need modern papers about operation of small hydro-power or run of river regards

12 September 2019 2,492 3 View

Is it possible to simulate a heterogeneous 3D architecture with GEM5 simulator ??

Is it possible to simulate a heterogeneous 3D architecture with GEM5 simulator ?? how can we create a layer of memories and a layer of processors ?

15 February 2019 2,511 1 View

Hello, I want to ask. Why is the thermodynamic calculations for some compounds not calculated by gaussian 09?

Hello I want to ask, What distinguishes the spectrum of nanoparticles ?

20 November 2018 8,960 3 View

Is it suitable to measure true density for oxide nanoparticles?

Is there a specified way to measure oxide Nano powder true density? if I have a known Nano powder weight is it possible to measure true density if I have a known liquid volume and the powder will...

15 March 2018 7,643 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View