Unable to separate well by centrifugation?

10 November 2018 0 895 Report

I put the V2O5/GO 0.1g (after calcination) into 50g of 95% ethanol and 50g of deionized water. It is difficult to pass the centrifugal separation again. Is there any reason or improvement?

Badges
Science method

More Han-Ching Jao's questions See All

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

Where do I download the codes, databases commonly used by researchers?EMG,EEG,etc?

Where do I download the codes, databases commonly used by researchers?

16 July 2024 295 0 View

How to download fasta files in "gene features" format from NCBI with multiple samples in R?

Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...

04 July 2024 9,875 1 View

Impact of nuclear-mitochondrial DNA segments (NUMTs) in phylogeny construction?

"Nuclear-mitochondrial DNA segments (NUMTs) are mitochondrial DNA (mtDNA) fragments that have been inserted into the nuclear genome." (Xue et al., 2023) I would like to know under this...

20 June 2024 603 1 View

How to transfect siRNA into C2C12 Myotubes?

Can Opti-MEM without serum be used for transfection? Myotubes are typically at a high density upon successful differentiation. Does this reduce the efficiency of transfection?

15 May 2024 5,843 0 View

For two Raman peaks belonging to the same irreducible representation, can one be polarized and the other depolarized?

If the depolarization ratio of a Raman peak is less than 0.75, it is a polarized band, and if it is greater than 0.75, it is a depolarized band.

04 May 2024 2,829 0 View

Does destaining buffer affect the primary antibody signal in the western blot?

After treating my membrane with the primary antibody, the destaining buffer was accidentally used instead of tbst buffer. Immediately after use (about 3 seconds later) it was replaced with tbst...

24 April 2024 2,625 1 View

Who knows what the membrane tension value of the OSCA channel is approximately?

For Mechanosensitive ion channel, I would like to compare their membrane tension. Most of their values can be found, but this one is not available.

15 April 2024 3,430 0 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

Need heterogeneous catalysts calcined before reduction?

Metal-containing heterogeneous catalysts often need to be calcined before reduction, so what are some examples of catalysts that perform better without calcination? What is the reason and how many...

05 August 2024 909 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

I have calcined my catalysts without flowing air from the compressed air cylinder will there be any problem with my reaction if i use that catalysts?

FYI Catalyst is MFI zeolite Si/Al = 140,25.40.11.5,15 I am using this catalyst to study methanol to DME conversion

25 July 2024 2,486 2 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View