Ultraviolet enzymatic method for BUN,

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Moaed E. Algazally Popular answer

thanks Maha Fahdil Mohammed for a nice comments and excellent answer.

Adam B Shapiro

Urease catalyzes the conversion of urea to ammonium ion and CO2.

Subsequently, glutamate dehydrogenase catalyzes the conversion of alpha-oxoglutarate, ammonium, and NADH to glutamate and NAD+.

The conversion of NADH to NAD results in a decrease in absorbance at 340 nm.

Moaed E. Algazally

BUN is most frequently measured by the urease –UV method in which the urease reaction is coupled to the glutamate dehydrogenase reaction,

generating NAD+.

Maha F. Smaism

the urease reaction is coupled to the glutamate dehydrogenase reaction,

Thanks Dr. Moaed Al-gazally for nice comments