Hi everyone,
I have been working on perfecting my OTCs for about six months. Things are going well but I am having trouble with sterile brushes. The brushes don't last long once sterilized and the brush fibres harden and sometimes tear the tissue which often renders them not viable.
My temporary solution has been to use a new brush for every batch but that is proving costly. Has your laboratory found a brush that is reusable for sensitive work or a brand of brushes that is cheaper and can be used as a standard? Or even a utensil that isn't a brush that works just as well.
Thanks
Hiya, by plating do you mean once you've first dissected the slices out, transferring them into a culture plate? I use a sterile glass dropper (sprayed with ethanol and held over a flame) to transfer my slices from the slicing medium to the insert in the culture plate. As the slice will still be in a drop of slicing buffer when you drop it onto the insert, I use a p200 pipette to gently suck this back up without touching the slice. Hope this helps!
Katie Stringer Thank you for your response! I am referring to once the slices have been placed in the inserts (we use small ones 1 slice per insert) we suck the slicing buffer out but the inserts are often a little bit sticking to the side, so we use a brush to centre them on the membrane. If not a brush, what do you use? Or do you use larger plates where it is unnecessary to arrange the slices on the membrane?
I use larger inserts that fit into a 6-well plate (3 slices per well), that gives them loads of room so you shouldn't need to arrange them. Good luck!
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