Type II membrane proteins are proteins spanning the membrane once, with its N-terminus on the cytoplasmic side of the membrane. The transmembrane domain is located close to the N-terminus and it functions as an anchor.
How does such a topology look if a type II protein is inserted in a subcellular organelle membrane (e.g. endoplasmic reticulum, Golgi, plastid)? Is the N-terminus exposed to the lumen and the C-terminus to the cytoplasm?
I would be grateful for some authoritative literature.
Hi Vladimir, it is the other way around: the N terminus is in the cytosol so the C terminus will be lumenal
You might find some links to specific structures from repositories like the Protein Data Bank and membrane protein-specific databases like Steve White's MPStruc db http://blanco.biomol.uci.edu/mpstruc/listAll/list . They would at least point you to the primary citations for such experimental structures.
You can take a look at this review for membrane protein insertion at endoplasmic reticulum http://www.ncbi.nlm.nih.gov/pubmed/21801011 it includes the type II membrane proteins
hi there.. while in most cases its the other way round.. there are a few exceptions where the C-terminal is in the cytosol and N-terminal acts as an anchor... one such example is the bacterial cell division protein ZipA
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