How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How can I maintain a surface at isothermal condition, say at 300 deg Celsius.?

I have 6 heaters each of power 0.5 kW and mounted below a curved mild steel sheet of thickness 4 mm. The heaters and the below section is covered and insulated. I want the top surface to be...

05 November 2020 199 5 View

What is the time required for an enzyme or an protein molecule to consume an ATP molecule during a reaction?

In what time do a protein body consumes an ATP molecule for an active process to happen?

02 October 2020 8,135 4 View

Person Temperature detection using thermal images taken from thermal camera?

I have tried based on pixel value with a referential ground-truth temperature of some different pixel value, a region of interest (thermal image of a person's forehead ). I am getting near about...

15 July 2020 336 1 View

How to calculate my research impact factor including first and second authorship?

If I have published x no of papers as a first author and y no of papers as the second author, how can I calculate my research impact factor not considering the citation? Thank you in advance

30 May 2020 5,086 17 View

What is the electrical conductivity and relative magnetic permeability of Steel P92 material ?

What is the electrical conductivity and relative magnetic permeability of Steel P92 material ?

25 February 2020 8,433 2 View

Is it possible to track endoplasmic reticulum and calcium present in that simultaneously by using dye? Do there exist any other alternative way ?

Is it possible to track endoplasmic reticulum and calcium present in that simultaneously by using dye? Do there exist any other alternative way ?

12 February 2020 9,022 3 View

How can we analyze cancer markers proteins in Co culture assay (PBMCs and Cancer cell)using WESTERN BLOTTING?

We are checking cancer cell death by activated PBMCs in a co-culture system. We specifically want to analyze the markers (Caspases, PI3K/mTor/AKT, BAX BCL2 and several other markers) using...

30 January 2020 7,697 2 View

How to correctly simulate deposition which shows induced defects and dislocations (if any due to deposition) in Silvaco ATHENA TCAD tool ?

I am trying to simulate the deposition of Silicon over SiO2. In reality, when we try to deposit a crystalline Silicon over SiO2 (amorphous), we get rather a polycrystalline Silicon. In ATHENA...

22 November 2019 4,916 4 View

How can I avoid any possible chances of getting algae in my rice hydroponics system?

I use light protected jars and big falcon tubes to grow my plants. Usually, I don't get algal contamination when growing plants for 2-3 weeks. But, sometimes I get algal growth in my samples. It...

23 June 2019 1,993 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to improve my BrdU immunostaining?

24 January 2021 414 3 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View

BrdU staining issue?

Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...

13 January 2021 9,449 0 View

How to do immunostaining with 3D culture (tissue embedded in matrigel)?

I dissected tissue from mice then embedded it in matrigel for culture. For analysis, I plan to do immunostaining with some cell markers. But the regular IF protocol did not work. Matrigel would...

11 January 2021 6,961 3 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View

CRISPR/Cas9 oligo design ideas and suggestions?

I need to design new oligonucleotides that can be used for gRNa/Cas9. The oligonucleotides need to target a gene from melangoster drosophila. Where do I start? How to find the gene to knock...

15 December 2020 4,729 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Can anyone help me in doing whole mount immunostaining on mouse colon?

I am exactly looking forward to know the procedure to isolate the myenteric and submucosal layer from intestinal whole mount. I would like to see the degeneration of dopaminergic neurons on it?...

12 October 2020 6,069 2 View