To expose an electrode with increasing concentrations of antigen, is PBS rinsing alone sufficient?

22 April 2014 1 2K Report

When testing a biosensor with different concentrations of antigen, it is said that the electrode should be flushed with PBS. Is PBS alone sufficient or the washing protocol with PBS+Tween 20 and PBS should be completed each time?

Shringika Soni

Doesn't Tween-20 will wash out the antibodies from electrode completely?

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