These ratios 260:230 and 260:280 are sufficient for carrying out a qPCR?

More Gustavo Moraes Ramos Valladão's questions See All

Journal of Research in Humanities and Social Science. Is a real magazine?

Dear colleagues: I have received many emails from a magazine called ournal of Research in Humanities and Social Science. I have gone to the website of this journal, and I read "some" indexes but,...

13 December 2020 6,208 6 View

How can I connect a thermocouple to an oscilloscope?

I would like to measure temperature with a very high acquisition rate during a test that takes place in under 1ms. I was thinking of using a thermocouple and an oscilloscope. Which kind of signal...

26 October 2020 1,736 6 View

How efficient are HiTrap desalting columns for separation of proteins from free fluorescent labels?

I am looking for a reliable and fast method for separation of protein-fluorescent probe conjugates from the respective free fluorophores present in labeling reactions. HiTrap desalting columns...

06 September 2020 8,039 8 View

PhyloCode now a mediatic debate?

The recent official publication of PhyloCode has caught my attention, not for new arguments being presented for a system that has already been extensively discussed and, as I see it, whose...

04 July 2020 726 10 View

How to write a password automatically in a bash script?

I have a script that download multiples files from a server, but for each one it requires me to write a password. These are 1000, so it would be laborious to write 1000 times the password. How...

02 April 2020 852 2 View

How to define parametric modulators for multiple conditions in SPM?

Dear RG Community, I am performing an SPM analysis in which I've got 8 conditions plus the baseline in an event-related experiment. Each condition has 4 trials, totalling 32 trials per run. There...

02 April 2020 7,060 1 View

Alkali Resistance of SS316, Nickel and Hastelloy?

Does anyone know or may anyone assist me to find which of these materials (Stainless Steel 316, Nickel, or Hastelloy) is the most resistant to a highly concentrated NaOH solution (>27 mol/L)...

26 March 2020 6,875 6 View

Recommendations/tips on publishing datasets?

In my research about vibration of hermetic compressors, I'm creating a dataset that I intend to make it available once I publish the paper it refers to. Do you have any tips or recommendations on...

18 March 2020 7,803 5 View

Tools for ncRNA in bacteria?

Hello, I am trying to determine if a region present in a Streptomyces genome acts as a ncRNA that affects gene expression. Which are the best tools to analyze sequences for this? Thanks!

24 November 2019 5,270 3 View

In Limma package, why should I linearize my data in some analyses?

tl;dr: Why data linearization is applied in the identification o differentially expressed genes/proteins? I am new to data analysis of big data like proteomics. As far as I know, a simple t-test...

16 November 2019 8,441 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View