We are determining the enantiomeric excess by NP-HPLC (using Chiralcel OD-H column employing Hexane:IPA solvent system). The product of racemic reaction show two peaks at Retention Time 18 and 20 min (each have 50% area). When we analyzed the same products (obtained from the reaction, in the presence of chiral catalyst) we obtained peaks with significant change in Retention Time (say at 23 and 25 min, having area 80% and 20%). Is this possible?

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