I analyze some allergenic dyes using LC-MS/MS and use 10mM ammonium acetate(0.1% FA) and ACN as mobile phase of LC-MS/MS.
However, there are some substances that are difficult to analyze.
Who know the MRM conditions (e.g. precursor ion, product ion) for substances down below?
I don't know which solvent I have to use and what is the most suitable condition for substances down below.
I'd appreciated it if anyone could tell me any advice.
Name / CAS
Solvent blue 4 / 6786-83-0
Please look at the following below links and the attached fille which may help you in your analysis:
https://www.shimadzu.eu/sites/shimadzu.seg/files/asms_2016_poster_lcms-8040_for_47_dyes_in_textiles.pdf
https://www2.mst.dk/Udgiv/publications/2014/04/978-87-93178-43-4.pdf
Thanks
We routinely analyze dyes (synthetic colors) by HPLC-UV. I have tried to move this analysis to LC-MS/MS without much luck. The dyes are quite sensitive using the UV since they absorb intensely in the visible spectrum, but certainly appear to not ionize reproducibly in ESI.
Also, with dyes you're better off with a neutral eluant. I would take out the FA; I don't think you need it. We run at a pH of 6.5 with an acetate buffer and unbuffered methanol.
Dyes are by definition visible (thus LC/UV)! Why use a shotgun (LC/MS/MS) to kill a fly?
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