How to run a mediation analysis with two different samples?

Hi, I tried to run a mediation analysis in which the DV is made up of test scores after a training in the experimental group VS the control group, and in which the mediator is measured by a...

17 November 2020 7,178 3 View

How can I add a chain identifier to a PDB file generated by Phyre2?

I am trying to use DynaMut2 to study stabilizing/destabilizing effects of mutations in a protein. I generated a 3D structure using Phyre2, and it gave me a PDB file without a chain identifier....

22 September 2020 495 11 View

Survey data collection sample purchase - Qualtrics or Dynata?

I am planning for a survey research project (national representative sample for 15 min survey) and am considering which panel/sampling company to use. I have used Dynata before, they are...

22 September 2020 9,334 4 View

Protocol for using Attune NxT Acoustic Focusing Cytometer to detect Ploidy level in leaf tissue?

Hello, I am looking for a detailed protocol to use the Attune NxT Acoustic Flow cytometer to determine ploidy level in plant samples with PI staining. Details for laser, threshold, and...

21 September 2020 8,334 2 View

Can a study have multiple research philosophies ?

I would like to know whether a study can have multiple research philosophy. I am currently studying the influence of ownership on firm performance through regression analysis. I am using case...

19 April 2020 1,073 3 View

How do you define the ontology and epistemology of mixed methods research?

The research which i am doing consists of multiple regression and case studies research. I would like to know whether what i am doing is a mixed methods. Also, what are its ontological and...

08 April 2020 5,457 15 View

Somebody know a fast DNA extraction method for plant for to do in the field?

I am looking found reference about people that has been working the proposed method in this publication: "Nucleic acid purification from plants, animals and microbes in under 30 seconds" from Zou...

10 March 2020 860 2 View

Is there any relevant literature detailing extraction methods of simple sugars from plant material for LC-MS analysis?

For my research, I would like to extract glucose, sucrose, and fructose from the crowns of strawberry plants and quantify these sugars using LC-MS. However, I have not had much luck finding a...

11 February 2020 398 3 View

How do I isolate LC nucleus for cell culture?

I need to isolate LC for primary cell culture? How should I do? What is better: use frozen tissue or fresh tissue?

21 January 2019 9,383 3 View

What happen if I put enzyme on optimum pH and temperature but I did not put any substrate to digest / hydrolyse?

I'm doing BSA test and I need a blank to identify the product of my substrate (which means I can't use water since BSA will read my enzyme as protein as well) ; problem is, once I substract the...

07 November 2018 5,006 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View