Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Pls inform me if the problem here is in the used red safe. im using the red safe of vivantis co, Malysia. using 5 microliter per 100 ml of agarose?

Why the ladder is always with so closle or attached bands after the 500 bp band whatever i use a type of ladder ( 5types) and also the PCR product is bended. is this has a relation with the used...

05 January 2021 5,211 5 View

Is it important/advisable to write a summary for each theme (and subthemes) in Thematic Analysis?

I am conducting semi-structured interviews with graduate students to explore their academic writing challenges. I am using thematic analysis to analyze the Qual findings. Should I include a...

31 December 2020 3,593 22 View

Using Matlab and the analytical solution for Room acoustic modeshapes, is it possible to plot modes in 3D?

mode shapes similar to attached image.

27 November 2020 4,262 7 View

What tool can I use for creating a Quantative Trait Loci map from SSR data?

I have SSR data for some individual of three hybride How I construct QTL mapping of yield according to these data ?

02 October 2020 502 5 View

Labs for purification, identification & isolation of unknown proteins form native sample of honey and to test versus GS-9L cells in vitro?

I asked for protein purification, identification and isolation of unknown proteins and test versus GS-9L other than that we already got in our patent WO/2014/040605 - form native sample of honey?!

23 September 2020 1,839 3 View

Hi everyone, do you know any GPS logger that we can use to conduct travel time surveys and which is easy to extract the data from ?

We are currently planning to conduct Freight Master Plan in the state of Qatar, one of the requirements is to conduct travel time surveys, therefore we are currently searching for GPS devices that...

22 July 2020 1,889 1 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

Any Predictive Markers for Severe COVID-19 in children?

Predictive Markers for Severe COVID-19 Identified

15 June 2020 1,375 5 View

How to critize (Evidence based medicine) this paper?

24 May 2020 5,489 3 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to increase mesh quality of a cfd model in ansys fluent?

How can one improve mesh skewness, orthogonality, aspect ratio?

28 February 2021 7,730 3 View

How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

Why is my CFD problem (mini heat sink rectangular channel) not converging while having good quality mesh?

My CFD problem is having two interconnected rectangular channel, which is known as mini heat sink. I have already performed parallel flow inside of an interconnected mini heat sink. However, I...

19 February 2021 3,209 3 View

How to compare means of continuous data with zero-inflation and skew?

I have two groups (unpaired) of plant biomass measurements and want to know whether there is a test that can be used to determine statistical significance where assumptions are not violated. The...

18 February 2021 1,258 4 View

T-test and Mann-Whitney U test on groups with different sample sizes and variances?

Hello I have 2 groups with different sample sizes of (experts: 5 to 8) and (Users: 28 to 35) answering 21 questions on a 7 points Likert scale about the importance of some dimensions for...

31 January 2021 3,885 7 View

Data Normality test in SPSS and Mardia's web of power to check skewness and kurtosis online test?

When we check our data set for univariate (e.g., SPSS) and Multivariate Skewness and Kurtosis as measures of non-normality as defined by Mardia (via Mardia' s web of Power) in order to...

12 January 2021 8,257 5 View

Bending moments in ABAQUS ?

Hello everyone !!!! I am modeling a cold formed steel profile in the ABAQUS program. I need to insert a non-uniform load into the structure section. Skew bending moments are applied at the two...

14 December 2020 7,138 2 View

Log reduction standard deviation bars on bar chart?

How do I create standard deviation bars for a log reduction graph? My st.dev seems to skew the graph as log reduction was not measured, only three repeats. Thankyou

10 December 2020 3,618 2 View