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How much nano second time study is required for study molecular dynamics in protein-protein complex to publish in journal?
04 August 2019 6,419 6 View
Can anyone send me the PyMol license file? Or, is there any other way to make high quality protein protein docked structure like PyMol?
04 August 2019 4,454 8 View
I am looking for Surface Plasmon Polariton excitation in gallium nano-sphere, so I wanted to know whether we should use plasma frequency of bulk material or nano structure effects the plasma...
27 January 2019 7,838 4 View
I am trying to transfect/ infect mouse podocyte cells (this is a cellline, but behaves very similarly like primary cells) with small DNAs. We tried using fugene. I am wondering if somebody can...
01 April 2016 8,932 5 View
is there any book available regarding close loop control of electical machine(dc m/c, PMSM, BLDC, IM, SM etc) which includes machine modelling along with control system design like we have...
31 January 2016 9,272 8 View
particle size distribution is pivotal as smaller the particle size greater will be the workability of the concrete .
04 January 2015 324 6 View
Selected area diffraction pattern does not come with intensity values. Can anyone here please suggest a software which can be used to calculate XRD-like patterns from it?
24 April 2014 7,502 2 View
I am looking forward to use in UV-SERS, Ga and Al are two prime candidate what can you comment on using Ga as plasmonics particle in 200-300nm range.
01 January 1970 5,101 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...
23 February 2021 7,051 4 View
I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...
23 February 2021 711 3 View
Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?
22 February 2021 2,968 1 View
