My peptide has strong interaction with SUMO, when I use 10 kDa molecular cut-off, the peptide also can not come through, just ask for help that any solution can break the interaction between peptide and SUMO (Solution was water before). Thanks
Dear Shiying Zhu ,
You mention strong interaction, so I presume that you mean it is related to a binding in a fashion as mentioned here:
Song, J., Durrin, L. K., Wilkinson, T. A., Krontiris, T. G., & Chen, Y. (2004). Identification of a SUMO-binding motif that recognizes SUMO-modified proteins. Proceedings of the National Academy of Sciences, 101(40), 14373-14378.
https://www.pnas.org/content/101/40/14373.long
See also (and references herein):
http://www.jbc.org/content/281/23/16117.full.pdf
It comes down to a region with hydrophobic and acidic amino acids as a SUMO-interacting motif (SIM).
Looking at some of the papers describing such interactions my educated guess would be to try a SDS buffer that presumable will break the interaction between the SUMO and your peptide.
Hope this will help you any further.
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