How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

What is the magnetic anisotropy constant for Permalloy (NiFe-80-20)? Can anyone suggest some papers?

Need to know the equation of anisotropy energy for permalloy and also the constants. Any reference paper will be helpful. Thanks for your time and cooperation.

26 January 2021 7,842 3 View

How to take into account the high resolution of the x-ray difractometer?

At small angles or in case high collimation, the peaks become anisotropic and with a Lorentz addition. The resolution describes correctly only with adding Lorentz and anisatropy parameters. It...

26 January 2021 5,829 4 View

What are the natural background values of Major,Trace and REE elements in sediments deposited by Indian rivers?

We are working on geochemical analysis of sediments derived from eastern and western ghats of India.

20 January 2021 8,963 6 View

What is the difference between soluble ions and exchangeable cations? And How to determine each of them?

I used percolation columns to reclamation saline-sodic clay soils . And my question is, What is the difference between soluble ions and exchangeable cations?

18 January 2021 6,868 3 View

Anionic sulfate complexes of rare earth elements, are they real?

Can you please tell me if there are anionic sulfate complexes of rare earth elements? And, if so, can you advise sources where you could read about it. Thank you!

17 January 2021 8,812 2 View

How to estimate total electron content using GPS data (with MATLAB)?

06 January 2021 1,110 2 View

Should you include the charge when writing out ions or ionic compounds?

I'm writing a chapter about the nitrogen cycle and discuss the different forms of reactive nitrogen such as NO3 and NH4. I also have some soil places where I discuss soil cations and others where...

22 December 2020 3,753 3 View

No title

I want to perform an interaction study between the Fe cation of a hemo group and an organic molecule. To do this, I want to use Grimme's dispersion correction with Becke-Johnson Damping (D3-BJ) to...

10 December 2020 2,067 3 View

Why contaminant is eluted with target proteins in cation exchange chromatography?

Me and my partner are doing project involving the purification of equine IgG using both anion and cation exchange chromatography. The main purpose of our project is to separate IgG from other...

10 November 2020 3,377 3 View